2KXM

Solution NMR Structure of the 27 nucleotide engineered neomycin sensing riboswitch RNA-ribostmycin complex

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	600
2	Bruker	AVANCE	Bruker Avance	700
3	Bruker	AVANCE	Bruker Avance	800
4	Bruker	AVANCE	Bruker Avance	900
5	Bruker	AVANCE	Bruker Avance	950

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-1H NOESY
10	3	4	2D DQF-COSY
11	3	4	2D 1H-1H NOESY
12	2	6	2D 1H-1H TOCSY
13	2	6	2D 1H-1H NOESY
2	2	2	2D 1H-15N HSQC
3	2	2	3D 1H-15N NOESY
4	3	3	2D 1H-13C HSQC
5	2	5	3D HNCO
6	2	5	3D H(CCO)NH
7	3	3	3D 1H-13C NOESY
8	3	3	3D 1H-13C NOESY
9	3	4	2D 1H-1H TOCSY

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.2		267-298
2	6.2		283
3	6.2		298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	10