2KTQ
OPEN TERNARY COMPLEX OF THE LARGE FRAGMENT OF DNA POLYMERASE I FROM THERMUS AQUATICUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Wavelength(s) | 0.98789,0.97938,0.97923,0.96859 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 108.414, 108.414, 89.843 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.300 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.28800 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 24.100 * |
| Refinement software | X-PLOR (3.851) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.000 |
| High resolution limit [Å] | 2.300 |
| Rmerge | 0.048 |
| Total number of observations | 319110 * |
| Number of reflections | 25623 |
| Completeness [%] | 93.4 |
| Redundancy | 12 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 20 * | pH 8.0 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | reservoir | HEPES | 0.1 (M) | |
| 2 | 1 | reservoir | 20 (mM) | ||
| 3 | 1 | reservoir | Na acetate | 0.1 (M) | |
| 4 | 1 | reservoir | PEG4000 | 10 (%(w/v)) |
