2KIN

KINESIN (MONOMERIC) FROM RATTUS NORVEGICUS

Experimental procedure

Source type	SYNCHROTRON
Source details	MPG/DESY, HAMBURG BEAMLINE BW6
Synchrotron site	MPG/DESY, HAMBURG
Beamline	BW6
Temperature [K]	90
Detector technology	IMAGE PLATE
Collection date	1996-08-28
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	71.556, 73.674, 74.128
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.000
R-factor	0.194 *
Rwork	0.194
R-free	0.25400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	UNREFINED KINESIN DIMER
RMSD bond length	0.017 *
RMSD bond angle	1.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.970
High resolution limit [Å]	1.900	1.900
Rmerge	0.048 *
Total number of observations	301422 *
Number of reflections	32107
<I/σ(I)>	13.6
Completeness [%]	99.3	98.8
Redundancy	5.1	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	19 *	HANGING DROP METHOD WAS USED. PROTEIN WAS CRYSTALLIZED FROM 20MM PIPES, PH 7.5, 50MM KCL, 1MM EGTA, 1MM DTT, 0.9 M LI2SO4. THE RESERVOIR CONTAINED 1.8 M LITHIUM SULFATE IN THE SAME BUFFER., vapor diffusion - hanging drop

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9-14 (mg/ml)
10	1	reservoir	EGTA	1 (mM)
11	1	reservoir	dithiothreitol	1 (mM)
2	1	drop	PIPES	20 (mM)
3	1	drop		50 (mM)
4	1	drop	EGTA	1 (mM)
5	1	drop	dithiothreitol	1 (mM)
6	1	drop	lithium sulfate	0.9 (M)
7	1	reservoir	lithium sulfate	1.8 (M)
8	1	reservoir	PIPES	20 (mM)
9	1	reservoir		50 (mM)