2KIF

Solution NMR structure of an O6-methylguanine DNA methyltransferase family protein from Vibrio parahaemolyticus. Northeast Structural Genomics Consortium target VpR247.

Spectrometer

Experimental method: SOLUTION NMR

Spectrometer ID	Spectrometer maker	Spectrometer model	Spectrometer type	Spectrometer field strength
1	Bruker	AVANCE	Bruker Avance	800
2	Bruker	AVANCE	Bruker Avance	600

Experiment

experiment id	conditions id	solution id	Experiment type
1	1	1	2D 1H-15N HSQC
10	1	2	3D HBHA(CO)NH
11	1	2	3D HCCH-COSY aliphatic
12	1	2	3D HCCH-TOCSY aliphatic
13	1	2	3D (H)CCH-TOCSY aliphatic
14	1	2	3D HNHA
15	1	2	3D HN(CO)CA
16	1	2	2D 1H-15N hetNOE
17	1	3	1D 1H-15N T1 and T2
18	1	3	2D 1H-13C HSQC high res. (L/V methyl stereoassignment)
2	1	1	2D 1H-13C HSQC
3	1	1	3D simultaneous CN NOESY
4	1	1	3D 1H-13C NOESY aromatic
5	1	2	3D HNCO
6	1	2	3D HN(CA)CO
7	1	2	3D HNCA
8	1	2	3D CBCA(CO)NH
9	1	2	3D HNCACB

NMR Sample

conditions id	NMR sample pH	NMR sample pressure	NMR sample temperature
1	6.5	ambient	298

Conformers

Conformers Calculated Total Number	100
Conformers Submitted Total Number	20