2JLT
Crystal structure of an RNA kissing complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-03 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 85.000, 85.000, 85.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 60.080 - 2.900 |
| R-factor | 0.189 |
| Rwork | 0.187 |
| R-free | 0.22700 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.468 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.5.0054) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.070 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.080 | 0.380 |
| Number of reflections | 10330 | |
| <I/σ(I)> | 18.66 | 5.45 |
| Completeness [%] | 98.9 | 96.6 |
| Redundancy | 6.43 | 6.09 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | NA CITRATE 100MM, MPD 35%, pH 4.6 |
