2JLN
Structure of Mhp1, a nucleobase-cation-symport-1 family transporter
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-02-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.700, 109.140, 113.820 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.014 - 2.850 |
| R-factor | 0.2362 |
| Rwork | 0.234 |
| R-free | 0.28090 |
| Structure solution method | MIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.480 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.950 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.120 | 0.770 |
| Number of reflections | 22414 | |
| <I/σ(I)> | 7.2 | 1 |
| Completeness [%] | 94.0 | 81 |
| Redundancy | 2.9 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | PROTEIN WAS CRYSTALLIZED FROM 31-33 % PEG 300, 100 MM NACL AND 100 MM NA-PHOSPHATE (PH 7.0), THAN SOAKED FOR 1 MINUTE IN 2.5 MM METHYL MERCURY ACETATE. |
