2JLI
Atomic resolution structure of the cytoplasmic domain of Yersinia pestis YscU, a regulatory switch involved in type III secretion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 48.162, 48.162, 190.204 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.700 - 1.130 |
| R-factor | 0.164 |
| Rwork | 0.164 |
| R-free | 0.16800 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.023 |
| RMSD bond angle | 2.232 |
| Data reduction software | HKL-3000 |
| Data scaling software | SCALEPACK |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.4.0057) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.160 |
| High resolution limit [Å] | 1.130 | 1.130 |
| Rmerge | 0.040 | 0.400 |
| Number of reflections | 49262 | |
| <I/σ(I)> | 50.4 | 6.2 |
| Completeness [%] | 97.6 | 91.1 |
| Redundancy | 13.5 | 11.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9.5 | 100 MM CHESS PH 9.5, 30% V/V PEG 400 |
