2JLF

STRUCTURAL EXPLANATION FOR THE ROLE OF MN IN THE ACTIVITY OF PHI6 RNA- DEPENDENT RNA POLYMERASE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 32
Unit cell lengths	110.040, 110.040, 159.132
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.021 - 3.200
R-factor	0.2304
Rwork	0.228
R-free	0.28600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hhs
RMSD bond length	0.000
RMSD bond angle	1.080
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	3.200
Rmerge	0.280
Number of reflections	52552
<I/σ(I)>	6
Completeness [%]	100.0
Redundancy	5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				15-20% POLYETHYLENE GLYCOL 4000, 8.5% ISOPROPANOL, 15% GLYCEROL AND 100MM HEPES, PH 7.5.