2JL9
Structural explanation for the role of Mn in the activity of phi6 RNA- dependent RNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SRS BEAMLINE PX9.6 |
Synchrotron site | SRS |
Beamline | PX9.6 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.519, 91.599, 142.705 |
Unit cell angles | 90.00, 101.64, 90.00 |
Refinement procedure
Resolution | 19.915 - 3.200 |
R-factor | 0.2177 |
Rwork | 0.215 |
R-free | 0.27200 |
Structure solution method | OTHER |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.280 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.300 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.180 | |
Number of reflections | 40323 | |
<I/σ(I)> | 7 | |
Completeness [%] | 91.0 | 81 |
Redundancy | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |