2JKV
Structure of human Phosphogluconate Dehydrogenase in complex with NADPH at 2.53A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-22 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 56.643, 204.714, 170.319 |
| Unit cell angles | 90.00, 90.65, 90.00 |
Refinement procedure
| Resolution | 36.899 - 2.532 |
| R-factor | 0.1566 |
| Rwork | 0.156 |
| R-free | 0.20690 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 1PGP 1pgn |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.069 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.670 |
| High resolution limit [Å] | 2.530 | 2.530 |
| Rmerge | 0.140 | 0.570 |
| Number of reflections | 128646 | |
| <I/σ(I)> | 7.8 | 2.1 |
| Completeness [%] | 97.9 | 87.1 |
| Redundancy | 3.7 | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.20M NA2SO4, 20.0% PEG 3350, 10.0% ETGLY |
