2JKU
Crystal structure of the N-terminal region of the biotin acceptor domain of human propionyl-CoA carboxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-06-21 |
Detector | MARRESEARCH |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 56.107, 56.107, 58.083 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.120 - 1.500 |
R-factor | 0.149 |
Rwork | 0.148 |
R-free | 0.16600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 2D5D 3bg3 |
RMSD bond length | 0.011 |
RMSD bond angle | 1.546 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.560 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.080 | 0.440 |
Number of reflections | 10251 | |
<I/σ(I)> | 23.6 | 5.2 |
Completeness [%] | 99.9 | 100 |
Redundancy | 12.7 | 13.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 20% PEG 3350, 0.20M NA FORMATE |