2JKU
Crystal structure of the N-terminal region of the biotin acceptor domain of human propionyl-CoA carboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-06-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 56.107, 56.107, 58.083 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.120 - 1.500 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.16600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ENTRIES 2D5D 3bg3 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.546 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0036) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.560 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.080 | 0.440 |
| Number of reflections | 10251 | |
| <I/σ(I)> | 23.6 | 5.2 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 12.7 | 13.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 20% PEG 3350, 0.20M NA FORMATE |
