2JKI
Complex of Hsp90 N-terminal and Sgt1 CS domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-19 |
Detector | ADSC CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 100.268, 129.654, 135.998 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 38.072 - 3.300 |
R-factor | 0.2009 |
Rwork | 0.199 |
R-free | 0.23830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB ENTRIES 1AMW 1rl1 |
RMSD bond length | 0.010 |
RMSD bond angle | 1.480 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 3.370 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.170 | 0.720 |
Number of reflections | 29660 | |
<I/σ(I)> | 7.9 | 1.7 |
Completeness [%] | 99.3 | 98 |
Redundancy | 3.5 | 3.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8.5 | 277 | INITIAL MULTIPLE CRYSTALS WERE GROWN BY VAPOR DIFFUSION AT 4 C AGAINST 26% W/V PEG4000, 100 MM TRIS (PH 8.5), AND 200 MM MAGNESIUM SULPHATE. SUBSEQUENT STREAK SEEDING INTO SOLUTIONS OF 16% W/V PEG4000, 100 MM TRIS (PH 8.5), AND 200 MM MAGNESIUM SULFATE PRODUCED SINGLE THIN PLATES. |