2JKD
Structure of the yeast Pml1 splicing factor and its integration into the RES complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-08-31 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 85.720, 85.720, 97.840 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.860 - 2.500 |
R-factor | 0.217 |
Rwork | 0.215 |
R-free | 0.26200 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.010 |
RMSD bond angle | 1.445 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.4.0065) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.600 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.038 | 0.225 |
Number of reflections | 14550 | |
<I/σ(I)> | 19.8 | 5.82 |
Completeness [%] | 98.2 | 99.2 |
Redundancy | 2.94 | 2.98 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.1M TRIS-HCL, PH 8.5, 0.2 M LISO4 AND FROM 20 TO 30% PEG 4000 |