2JK8
Type IV secretion system effector protein BepA complexed with a pyrophosphate moiety
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.948, 82.877, 126.729 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.800 |
| R-factor | 0.218 |
| Rwork | 0.216 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vy3 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.189 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 82.760 | 2.950 |
| High resolution limit [Å] | 2.730 | 2.800 |
| Rmerge | 0.100 | 0.440 |
| Number of reflections | 17907 | |
| <I/σ(I)> | 5.6 | 1.62 |
| Completeness [%] | 91.9 | 55.9 |
| Redundancy | 3.45 | 2.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.7 | BETA-MERCAPTOETHANOL MIXED WITH 22 % POLYETHYLENE GLYCOL 4000, 0.1 M TRIS PH 8.7, 5 MM NICL2 IN A 1:1 (V:V) RATIO |
