2JK5
Potassium Channel KcsA in complex with Tetrabutylammonium in high K
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-11-19 |
Detector | MARRESEARCH |
Spacegroup name | I 4 |
Unit cell lengths | 156.145, 156.145, 76.326 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.400 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.24900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1K4C WITH SELECTIVITY FILTER AND IONS REMOVED |
RMSD bond length | 0.011 |
RMSD bond angle | 1.291 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0051) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.540 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.070 | 0.440 |
Number of reflections | 35756 | |
<I/σ(I)> | 15.27 | 3.5 |
Completeness [%] | 99.5 | 98.9 |
Redundancy | 3.75 | 3.75 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 5.4 | 18-25%PEG400, 50 MM SODIUM ACETATE, 50 MM MAGNESIUM ACETATE, pH 5.4 |