2JK0

Structural and functional insights into Erwinia carotovora L- asparaginase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	73.650, 135.630, 250.100
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.500
R-factor	0.192
Rwork	0.188
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hg1
RMSD bond length	0.009
RMSD bond angle	1.292
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.600
High resolution limit [Å]	2.500	2.500
Rmerge	0.080	0.440
Number of reflections	83599
<I/σ(I)>	14	3.2
Completeness [%]	95.8	78
Redundancy	4.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1