2JJM
Crystal Structure of a family GT4 glycosyltransferase from Bacillus anthracis ORF BA1558.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 290 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 134.597, 204.667, 135.329 |
| Unit cell angles | 90.00, 115.49, 90.00 |
Refinement procedure
| Resolution | 20.000 - 3.100 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.26200 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 3.270 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.110 | 0.870 |
| Number of reflections | 117619 | |
| <I/σ(I)> | 2.1 | |
| Completeness [%] | 98.5 | 98 |
| Redundancy | 7.5 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.1M HEPES PH 7.5, 0.2M NA2SO4, 14% PEG 3350 |
