2JJM
Crystal Structure of a family GT4 glycosyltransferase from Bacillus anthracis ORF BA1558.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-4 |
Synchrotron site | ESRF |
Beamline | ID14-4 |
Temperature [K] | 290 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 134.597, 204.667, 135.329 |
Unit cell angles | 90.00, 115.49, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.100 |
R-factor | 0.254 |
Rwork | 0.254 |
R-free | 0.26200 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.019 |
RMSD bond angle | 1.900 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SHELX |
Refinement software | CNS (1.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 3.270 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.110 | 0.870 |
Number of reflections | 117619 | |
<I/σ(I)> | 2.1 | |
Completeness [%] | 98.5 | 98 |
Redundancy | 7.5 | 7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 0.1M HEPES PH 7.5, 0.2M NA2SO4, 14% PEG 3350 |