2JJ2
The Structure of F1-ATPase inhibited by quercetin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 106.399, 282.019, 138.093 |
| Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
| Resolution | 80.580 - 2.400 |
| R-factor | 0.19 |
| Rwork | 0.188 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1w0j |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.162 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 80.580 | 2.530 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.080 | 0.400 |
| Number of reflections | 297401 | |
| <I/σ(I)> | 7.2 | 2.3 |
| Completeness [%] | 94.3 | 88.1 |
| Redundancy | 2.1 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.2 | pH 8.2 |
