2JJ2
The Structure of F1-ATPase inhibited by quercetin.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-1 |
Synchrotron site | ESRF |
Beamline | ID14-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.399, 282.019, 138.093 |
Unit cell angles | 90.00, 90.44, 90.00 |
Refinement procedure
Resolution | 80.580 - 2.400 |
R-factor | 0.19 |
Rwork | 0.188 |
R-free | 0.23800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w0j |
RMSD bond length | 0.009 |
RMSD bond angle | 1.162 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.580 | 2.530 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.080 | 0.400 |
Number of reflections | 297401 | |
<I/σ(I)> | 7.2 | 2.3 |
Completeness [%] | 94.3 | 88.1 |
Redundancy | 2.1 | 2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | pH 8.2 |