2JJ1
The Structure of F1-ATPase inhibited by piceatannol.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 106.957, 281.176, 138.785 |
Unit cell angles | 90.00, 89.58, 90.00 |
Refinement procedure
Resolution | 72.740 - 2.700 |
R-factor | 0.205 |
Rwork | 0.202 |
R-free | 0.26900 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1w0j |
RMSD bond length | 0.010 |
RMSD bond angle | 1.263 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 |
Phasing software | AMoRE |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.360 | 2.850 |
High resolution limit [Å] | 2.700 | 2.700 |
Rmerge | 0.090 | 0.350 |
Number of reflections | 201334 | |
<I/σ(I)> | 7.3 | 1.9 |
Completeness [%] | 90.3 | 92.5 |
Redundancy | 1.4 | 1.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.2 | pH 8.2 |