2JIY

PHOTOSYNTHETIC REACTION CENTER MUTANT WITH ALA M149 REPLACED WITH TRP (CHAIN M, AM149W)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX14.1
Synchrotron site	SRS
Beamline	PX14.1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 31 2 1
Unit cell lengths	139.785, 139.785, 185.454
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	15.970 - 2.200
R-factor	0.183
Rwork	0.181
R-free	0.20700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	WILD-TYPE RHODOBACTER SPHAEROIDES COORDINATES UNPUBLISHED DATA
RMSD bond length	0.010
RMSD bond angle	1.360
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.900	2.250
High resolution limit [Å]	2.200	2.200
Rmerge	0.070	0.570
Number of reflections	106556
<I/σ(I)>	20.6	2.7
Completeness [%]	98.6	98.4
Redundancy	3.8	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1