2JIG

Crystal structure of Chlamydomonas reinhardtii prolyl-4 hydroxylase type I complexed with zinc and pyridine-2,4-dicarboxylate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X12
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X12
Temperature [K]	100
Detector technology	CCD
Collection date	2006-12-14
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	57.920, 60.250, 116.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.170 - 1.850
R-factor	0.186
Rwork	0.183
R-free	0.23800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2v4a
RMSD bond length	0.011
RMSD bond angle	1.241
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.3.0028)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.100	0.320
Number of reflections	152984
<I/σ(I)>	6.3	2.7
Completeness [%]	98.1	89.2
Redundancy	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1