2JI5
Structure of UMP kinase from Pyrococcus furiosus complexed with UTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM16 |
| Synchrotron site | ESRF |
| Beamline | BM16 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-10-03 |
| Detector | MARRESEARCH |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 144.943, 144.943, 144.943 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.000 - 2.450 |
| R-factor | 0.2094 |
| Rwork | 0.209 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bmu |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.460 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.550 | 2.580 |
| High resolution limit [Å] | 2.450 | 2.450 |
| Rmerge | 0.090 | 0.410 |
| Number of reflections | 9813 | |
| <I/σ(I)> | 22.4 | 4.9 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 9.3 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 3.5 M SODIUM FORMATE, pH 8 |
