2JI0
CRYSTAL STRUCTURE OF RHOGDI K138Y, K141Y MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR591 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2005-09-05 |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.013, 55.124, 38.935 |
| Unit cell angles | 90.00, 107.50, 90.00 |
Refinement procedure
| Resolution | 37.140 - 2.100 |
| R-factor | 0.188 |
| Rwork | 0.184 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 3.077 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 2.180 | 2.180 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.040 | 0.170 |
| Number of reflections | 5179 | |
| <I/σ(I)> | 18.6 | 3.91 |
| Completeness [%] | 67.5 | 22.5 |
| Redundancy | 1.7 | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 32% PEG 8000, 0.22M AMMONIUM SULFATE, 0.1 M SODIUM CACODYLATE PH6.5, pH 7.50 |
