2JHZ
CRYSTAL STRUCTURE OF RHOGDI E155S, E157S MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-07 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 130.629, 130.629, 163.409 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 92.850 - 2.200 |
| R-factor | 0.188 |
| Rwork | 0.186 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxw |
| RMSD bond length | 0.013 |
| RMSD bond angle | 3.175 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.090 | 0.200 |
| Number of reflections | 27390 | |
| <I/σ(I)> | 31.4 | 6.3 |
| Completeness [%] | 99.6 | 97.3 |
| Redundancy | 7.8 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5 |
