2JHY
CRYSTAL STRUCTURE OF RHOGDI E155H, E157H MUTANT
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 75.221, 75.221, 91.556 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 65.090 - 1.900 |
| R-factor | 0.226 |
| Rwork | 0.225 |
| R-free | 0.25000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bxw |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.845 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.620 |
| Number of reflections | 23942 | |
| <I/σ(I)> | 32.1 | 1.8 |
| Completeness [%] | 99.4 | 94.3 |
| Redundancy | 10.1 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.4 M SODIUM CITRATE 0.1 M HEPES PH 7.5 |
