2JHR
Crystal structure of myosin-2 motor domain in complex with ADP- metavanadate and pentabromopseudilin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-04-19 |
Detector | Bruker Platinum 135 |
Wavelength(s) | 1.54 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 89.758, 150.464, 154.550 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 8.000 - 2.800 |
R-factor | 0.217 |
Rwork | 0.217 |
R-free | 0.26500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1yv3 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.400 |
Data reduction software | PROTEUM2 |
Data scaling software | PROTEUM2 |
Phasing software | AMoRE |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.090 | 0.400 |
Number of reflections | 25708 | |
<I/σ(I)> | 10.9 | 2.4 |
Completeness [%] | 98.2 | 95.5 |
Redundancy | 5.2 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.4 | 50 MM HEPES PH 7.4, 140 MM NACL, 11% W/V PEG8000, 2% (V/V) MPD, 5 MM MGCL2, 5 MM DTT, 1 MM EGTA. |