2JHC
The structure of bluetongue virus VP4 reveals a multifunctional RNA- capping production-line
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-2 |
Synchrotron site | ESRF |
Beamline | ID14-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-06-20 |
Detector | ADSC CCD |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 75.239, 75.239, 421.838 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.000 - 3.000 |
R-factor | 0.258 |
Rwork | 0.254 |
R-free | 0.33300 |
Structure solution method | OTHER |
RMSD bond length | 0.023 |
RMSD bond angle | 1.884 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.000 | 3.110 |
High resolution limit [Å] | 3.000 | 3.000 |
Rmerge | 0.160 | 0.540 |
Number of reflections | 14461 | |
<I/σ(I)> | 16 | 1.1 |
Completeness [%] | 99.7 | 99.8 |
Redundancy | 14.6 | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.7 | 100 MM IMIDAZOLE PH 8.0, 2%-8% PROPAN-2-OL, 0-250 MM NACL, 50 MM DTT |