2JGU
crystal structure of DNA-directed DNA polymerase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC CCD |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 92.178, 127.923, 89.210 |
Unit cell angles | 90.00, 109.11, 90.00 |
Refinement procedure
Resolution | 15.000 - 2.600 |
R-factor | 0.2749 |
Rwork | 0.275 |
R-free | 0.27110 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1qqc |
RMSD bond length | 0.015 |
RMSD bond angle | 2.334 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | CNS (1.1) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 2.690 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.060 | 0.260 |
Number of reflections | 848408 | |
<I/σ(I)> | 18.4 | 1.72 |
Completeness [%] | 93.8 | 85.1 |
Redundancy | 4.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | pH 6.5 |