2JG3
MtaqI with BAZ
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2001-04-21 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.404, 69.193, 114.390 |
| Unit cell angles | 90.00, 92.16, 90.00 |
Refinement procedure
| Resolution | 19.730 - 1.900 |
| R-factor | 0.187 |
| Rwork | 0.185 |
| R-free | 0.22600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1g38 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.699 |
| Data reduction software | MAR |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.790 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.090 | 0.310 |
| Number of reflections | 72769 | |
| <I/σ(I)> | 14.62 | 4.94 |
| Completeness [%] | 99.4 | 97.7 |
| Redundancy | 4.522 | 2.88 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.3 | 298 | 3 MICROLITERS CRYSTALLIZATION BUFFER (10 MM TRIS/HCL, 300 MM NACL, PH 7.3) CONTAINING THE COMPLEX PLUS 1 MICROLITER RESERVOIR SOLUTION (100 MM KCL, 100 MM MGCL2, 6% ISOPROPANOL, 50 MM SODIUM CACODYLATE, PH 6.0), VAPOR DIFFUSION, HANGING DROP, TEMPERATURE 298K |
