2JFW
Crystal structure of Enterococcus faecium glutamate racemase in complex with tartrate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-05-11 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 85.423, 85.423, 93.790 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 22.000 - 2.000 |
Rwork | 0.204 |
R-free | 0.24480 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jfu |
RMSD bond length | 0.056 |
RMSD bond angle | 1.330 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | CNX (2000.2) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 22.000 | 2.100 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.070 | 0.560 |
Number of reflections | 25692 | |
<I/σ(I)> | 7.6 | 1.7 |
Completeness [%] | 94.6 | 78.7 |
Redundancy | 3.3 | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | PROTEIN FORMULATED AT 10 MG/ML WITH 200MM AMMONIUM ACETATE PH 7.4, 5MM D-L GLUTAMATE, 1 MM TCEP AND CRYSTALLISED WITH 200 MM DI-SODIUM TATRATE AND 25% PEG 3350 |