2JFP
Crystal structure of Enterococcus faecalis glutamate racemase in complex with D- Glutamate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-05-28 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.190, 74.670, 75.070 |
| Unit cell angles | 90.00, 95.97, 90.00 |
Refinement procedure
| Resolution | 34.000 - 1.980 |
| Rwork | 0.204 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jfo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.272 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | CNX (2002.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.000 | 2.080 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.070 | 0.250 |
| Number of reflections | 36578 | |
| <I/σ(I)> | 4.9 | 2.5 |
| Completeness [%] | 97.9 | 86.7 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN FORMULATED AT 10 MG/ML WITH 200MM AMMONIUM ACETATE PH 7.4, 5MM D-L GLUTAMATE, 1 MM TCEP AND CRYSTALLISED WITH 0.1 M TRIS PH 7.5 0.2 MM CACL2 AND 20-25% PEG 3350 |
