2JFD
Structure of the MAT domain of human FAS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-06 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 85.980, 92.690, 259.870 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.220 - 2.810 |
| R-factor | 0.215 |
| Rwork | 0.212 |
| R-free | 0.27300 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.258 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.300 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.050 | 0.410 |
| Number of reflections | 51294 | |
| <I/σ(I)> | 15.8 | 2.39 |
| Completeness [%] | 98.4 | 90.5 |
| Redundancy | 4 | 3.35 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 20% PEG10K, 0.04 M NAKPO4, 25% GLYCEROL, pH 8.50 |
