2JF3
Nucleotide substrate binding by UDP-N-acetylglucosamine acyltransferase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-05-20 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 3 |
| Unit cell lengths | 97.740, 97.740, 97.740 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 69.170 - 3.000 |
| R-factor | 0.22 |
| Rwork | 0.218 |
| R-free | 0.27000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lxa |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.940 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 69.170 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.110 | 0.340 |
| Number of reflections | 6451 | |
| <I/σ(I)> | 7.6 | 2.5 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 3.2 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10% PEG 10000, 0.1M MES PH6.5, pH 6.50 |
