2JEQ
Family 5 xyloglucanase from Paenibacillus pabuli in complex with ligand
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-08-04 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 85.066, 89.160, 38.705 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 61.550 - 1.940 |
| R-factor | 0.186 |
| Rwork | 0.182 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jep |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.443 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.110 | 0.420 |
| Number of reflections | 22170 | |
| <I/σ(I)> | 13.3 | 3 |
| Completeness [%] | 98.8 | 91.7 |
| Redundancy | 4.2 | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 25% PEG 3350, 0.2 M MGCL2, 0.1 M TRIS, PH 8.5 |
