2JEM
Native family 12 xyloglucanase from Bacillus licheniformis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 120 |
| Detector technology | IMAGE PLATE |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 48.748, 78.133, 69.103 |
| Unit cell angles | 90.00, 109.80, 90.00 |
Refinement procedure
| Resolution | 64.550 - 1.780 |
| R-factor | 0.156 |
| Rwork | 0.155 |
| R-free | 0.20000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nlr |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.360 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.820 |
| High resolution limit [Å] | 1.780 | 1.780 |
| Rmerge | 0.040 | 0.250 |
| Number of reflections | 44098 | |
| <I/σ(I)> | 27.7 | 4.1 |
| Completeness [%] | 94.3 | 81.5 |
| Redundancy | 4.1 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 10% W/V PEG4000, 0.05 M MGCL2, 0.1 M BIS-TRIS, PH 6.5 |
