2JEM
Native family 12 xyloglucanase from Bacillus licheniformis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RU200 |
Temperature [K] | 120 |
Detector technology | IMAGE PLATE |
Detector | MARRESEARCH |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.748, 78.133, 69.103 |
Unit cell angles | 90.00, 109.80, 90.00 |
Refinement procedure
Resolution | 64.550 - 1.780 |
R-factor | 0.156 |
Rwork | 0.155 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1nlr |
RMSD bond length | 0.013 |
RMSD bond angle | 1.360 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Refinement software | REFMAC (5.3.0011) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.820 |
High resolution limit [Å] | 1.780 | 1.780 |
Rmerge | 0.040 | 0.250 |
Number of reflections | 44098 | |
<I/σ(I)> | 27.7 | 4.1 |
Completeness [%] | 94.3 | 81.5 |
Redundancy | 4.1 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 10% W/V PEG4000, 0.05 M MGCL2, 0.1 M BIS-TRIS, PH 6.5 |