Experimental procedure
| Source type | ROTATING ANODE |
| Source details | ENRAF-NONIUS FR571 |
| Temperature [K] | 289 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1992-04 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.850, 67.380, 77.090 |
| Unit cell angles | 90.00, 97.34, 90.00 |
Refinement procedure
| Resolution | 6.000 - 2.500 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | GLOOP2 FAB |
| RMSD bond length | 0.006 |
| RMSD bond angle | 26.000 * |
| Data reduction software | Agrovata ((CCP4)) |
| Data scaling software | Agrovata |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 2.560 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.046 * | 0.190 * |
| Total number of observations | 47817 * | |
| Number of reflections | 22067 | 1043 * |
| <I/σ(I)> | 11 | 3.5 |
| Completeness [%] | 96.6 | 93 |
| Redundancy | 2.7 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.8 | the molar ratio of Fab fragment to HPr was approximately 1:2 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | Fab fragment | 10 (mg/ml) | |
| 2 | 1 | drop | HPr | 4 (mg/ml) | |
| 3 | 1 | drop | ammonium sulfate | 25 (%sat) | |
| 4 | 1 | drop | phosphate | 25 (mM) |
