2JDY
Mutant (G24N) of Pseudomonas aeruginosa lectin II (PA-IIL) complexed with methyl-b-D-mannoyranoside
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-04-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.383, 80.085, 52.493 |
| Unit cell angles | 90.00, 109.55, 90.00 |
Refinement procedure
| Resolution | 49.450 - 1.700 |
| R-factor | 0.143 |
| Rwork | 0.142 |
| R-free | 0.17300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1uzv |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.257 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.450 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.050 | 0.200 |
| Number of reflections | 42206 | |
| <I/σ(I)> | 20.1 | 6.6 |
| Completeness [%] | 97.8 | 88.7 |
| Redundancy | 3.5 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 0.1 M HEPES (PH 7.5), 10% ISO-PROPANOL, 20% PEG 4000. |
