2JDV
Structure of PKA-PKB chimera complexed with A-443654
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Temperature [K] | 100 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 72.809, 74.788, 80.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 35.400 - 2.080 |
| R-factor | 0.21 |
| Rwork | 0.206 |
| R-free | 0.27600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yds |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.505 |
| Refinement software | REFMAC (5.2.0019F) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Rmerge | 0.050 | 0.230 |
| Number of reflections | 24115 | |
| <I/σ(I)> | 10.8 | 3.1 |
| Completeness [%] | 94.0 | 87 |
| Redundancy | 2.3 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
