Loading
PDBj
MenuPDBj@FacebookPDBj@TwitterPDBj@YouTubewwPDB FoundationwwPDB
RCSB PDBPDBeBMRBAdv. SearchSearch help

2JDQ

C-terminal domain of influenza A virus polymerase PB2 subunit in complex with human importin alpha5

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeSYNCHROTRON
Source detailsESRF BEAMLINE ID23-1
Synchrotron siteESRF
BeamlineID23-1
Temperature [K]100
Detector technologyCCD
Collection date2006-06-15
DetectorADSC CCD
Spacegroup nameP 21 21 21
Unit cell lengths94.970, 100.550, 151.840
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution30.000 - 2.200
R-factor0.208
Rwork0.206
R-free0.24700
Structure solution methodMOLECULAR REPLACEMENT
Starting model (for MR)1q1s
RMSD bond length0.012
RMSD bond angle1.273
Data reduction softwareXDS
Data scaling softwareXSCALE
Phasing softwarePHASER
Refinement softwareREFMAC (5.2.0019)
Data quality characteristics
 OverallOuter shell
Low resolution limit [Å]50.0002.300
High resolution limit [Å]2.2002.200
Rmerge0.0900.690
Number of reflections74104
<I/σ(I)>9.42.4
Completeness [%]99.599.9
Redundancy44.06
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
14.61 MICROLITRE OF PROTEIN SOLUTION, AT 20 MG/ML IN 30 MM TRIS-HCL, PH 7.5, 150 MM NACL, 3 MM BETA-MERCAPTOETHANOL WAS MIXED WITH AN EQUAL VOLUME OF RESERVOIR SOLUTION (0.1 M NAAC, PH 4.6, 5 MM CACL2, 15% MPD)

227111

PDB entries from 2024-11-06

PDB statisticsPDBj update infoContact PDBjnumon