2JDL
Structure of C-terminal region of acidic P2 ribosomal protein complexed with trichosanthin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 62.589, 43.964, 92.235 |
| Unit cell angles | 90.00, 92.87, 90.00 |
Refinement procedure
| Resolution | 25.470 - 2.200 |
| R-factor | 0.167 |
| Rwork | 0.163 |
| R-free | 0.23800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tcs |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.068 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 92.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.100 | 0.260 |
| Number of reflections | 24055 | |
| <I/σ(I)> | 9.6 | 3.2 |
| Completeness [%] | 99.4 | 98 |
| Redundancy | 2.4 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7% PEG 20000, 0.1M MES, PH 6.5 |
