2JDK
Lectin PA-IIL of P.aeruginosa complexed with disaccharide derivative
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-12 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.611, 73.078, 54.501 |
| Unit cell angles | 90.00, 94.35, 90.00 |
Refinement procedure
| Resolution | 54.310 - 1.100 |
| R-factor | 0.113 |
| Rwork | 0.112 |
| R-free | 0.13400 |
| Structure solution method | OTHER |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.686 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 22.300 |
| High resolution limit [Å] | 1.100 |
| Rmerge | 0.050 |
| Number of reflections | 165425 |
| <I/σ(I)> | 18.7 |
| Completeness [%] | 99.4 |
| Redundancy | 5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | TRIS HCL 0.1M, PH8.5, 1.75 M AMMNONIUM SULFATE(THE PA-IIL/GLYCOMIMETIC COMPLEXE WAS OBTAINED BY SOAKING METHOD FROM FUCOSE-CONTAINING CRISTAL) |
