2JDJ
crystal structure of HapK from Hahella chejuensis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-05 |
Detector | ADSC CCD |
Spacegroup name | P 32 1 2 |
Unit cell lengths | 58.306, 58.306, 137.882 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 28.520 - 2.000 |
R-factor | 0.191 |
Rwork | 0.189 |
R-free | 0.22300 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.305 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | SOLVE |
Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.070 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.080 | 0.290 |
Number of reflections | 35383 | |
<I/σ(I)> | 44 | 8.6 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11 | 11 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 6.5 | 1.8 M AMMONIUM SULFATE, 7% ISOPROPANOL, pH 6.5 |