2JDC
Glyphosate N-acetyltransferase bound to oxidized COA and sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-06-04 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 69.056, 48.911, 46.461 |
| Unit cell angles | 90.00, 103.54, 90.00 |
Refinement procedure
| Resolution | 32.160 - 1.600 |
| R-factor | 0.149 |
| Rwork | 0.148 |
| R-free | 0.17200 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 2bsw |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.449 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 32.200 | 1.660 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Rmerge | 0.020 | 0.060 |
| Number of reflections | 19217 | |
| <I/σ(I)> | 47.9 | 19 |
| Completeness [%] | 96.0 | 75.4 |
| Redundancy | 3.3 | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.6 | 100 MM SODIUM ACETATE, PH 4.6 150-300 MM AMMONIUM SULFATE 20-25% PEG4000 2 MM ACETYL COA |
