2JD9

Structure of a pectin binding carbohydrate binding module determined in an orthorhombic crystal form.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	113
Spacegroup name	P 21 21 21
Unit cell lengths	34.765, 42.630, 98.681
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 1.800
R-factor	0.175
Rwork	0.172
R-free	0.24000
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.021
RMSD bond angle	1.875
Phasing software	MOLREP
Refinement software	REFMAC (5.1.24)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.860
High resolution limit [Å]	1.800	1.800
Rmerge	0.080	0.350
Number of reflections	12978
<I/σ(I)>	20.3	6.8
Completeness [%]	96.2	53.5
Redundancy	14	13.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1