2JD8

Crystal Structure of the Zn-soaked Ferritin from the Hyperthermophilic Archaeal Anaerobe Pyrococcus furiosus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	CCD
Collection date	2006-06-25
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	255.371, 342.055, 265.986
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	204.120 - 2.800
R-factor	0.203
Rwork	0.200
R-free	0.25100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2jd6
RMSD bond length	0.015
RMSD bond angle	1.479
Data reduction software	MOSFLM
Data scaling software	SCALA
Refinement software	REFMAC (5.3.0021)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.900	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.080	0.290
Number of reflections	279537
<I/σ(I)>	8.8	2.5
Completeness [%]	98.7	91.6
Redundancy	3.9	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				THE CRYSTALS USED FOR DATA COLLECTION WERE GROWN AGAINST 0.5 ML OF 2 M AMMONIUM SULPHATE RESERVOIR SOLUTION, WITH DROPS CONTAINING 2 MICROL ITERS OF 5 MG/ML PROTEIN SOLUTION IN 50 MM HEPES, PH 7 AND EQUAL AMOUNT OF THE RESERVOIR SOLUTION. ZINC SOAKING OF FERRITIN CRYSTALS WAS PERFORMED BY TRANSFERRING THEM TO A 10-20 MICROLITER DROP OF A SOLUTION WITH COMPOSITION 20 MM ZNCL2, 25 % GLYCEROL AND 2 M AMMONIUM SULFATE, FOR 15 MINUTES.