2JD8
Crystal Structure of the Zn-soaked Ferritin from the Hyperthermophilic Archaeal Anaerobe Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-06-25 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 255.371, 342.055, 265.986 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 204.120 - 2.800 |
R-factor | 0.203 |
Rwork | 0.200 |
R-free | 0.25100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jd6 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.479 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.900 | 2.950 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.080 | 0.290 |
Number of reflections | 279537 | |
<I/σ(I)> | 8.8 | 2.5 |
Completeness [%] | 98.7 | 91.6 |
Redundancy | 3.9 | 3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | THE CRYSTALS USED FOR DATA COLLECTION WERE GROWN AGAINST 0.5 ML OF 2 M AMMONIUM SULPHATE RESERVOIR SOLUTION, WITH DROPS CONTAINING 2 MICROL ITERS OF 5 MG/ML PROTEIN SOLUTION IN 50 MM HEPES, PH 7 AND EQUAL AMOUNT OF THE RESERVOIR SOLUTION. ZINC SOAKING OF FERRITIN CRYSTALS WAS PERFORMED BY TRANSFERRING THEM TO A 10-20 MICROLITER DROP OF A SOLUTION WITH COMPOSITION 20 MM ZNCL2, 25 % GLYCEROL AND 2 M AMMONIUM SULFATE, FOR 15 MINUTES. |