2JD6
Crystal Structure of the as isolated Ferritin from the Hyperthermophilic Archaeal Anaerobe Pyrococcus furiosus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM14 |
Synchrotron site | ESRF |
Beamline | BM14 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-06-24 |
Detector | MARRESEARCH |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 254.097, 341.422, 265.524 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 204.120 - 2.750 |
R-factor | 0.198 |
Rwork | 0.195 |
R-free | 0.24700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vlg |
RMSD bond length | 0.014 |
RMSD bond angle | 1.432 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.3.0021) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.200 | 2.900 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.080 | 0.300 |
Number of reflections | 295335 | |
<I/σ(I)> | 16.5 | 3.1 |
Completeness [%] | 99.7 | 97.8 |
Redundancy | 5.7 | 3.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | THE CRYSTALS USED FOR DATA COLLECTION WERE GROWN AGAINST 0.5 ML OF 2 M AMMONIUM SULPHATE RESERVOIR SOLUTION, WITH DROPS CONTAINING 2 MICRO-L OF 5 MG/ML PROTEIN SOLUTION IN 50 MM HEPES, PH 7 AND EQUAL AMOUNT OF THE RESERVOIR SOLUTION. |