2JCW

REDUCED BRIDGE-BROKEN YEAST CU/ZN SUPEROXIDE DISMUTASE ROOM TEMPERATURE (298K) STRUCTURE

Replaces:  1JCW

Experimental procedure

Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	298
Detector technology	IMAGE PLATE
Collection date	1993-10
Detector	RIGAKU
Spacegroup name	H 3 2
Unit cell lengths	119.280, 119.280, 75.050
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	10.000 - 1.700
R-factor	0.191
Rwork	0.191
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sdy
RMSD bond length	0.009
RMSD bond angle	25.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.1)
Refinement software	X-PLOR (3.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.000	1.760
High resolution limit [Å]	1.700	1.700
Rmerge	0.099 *
Number of reflections	19256
Completeness [%]	82.3	40.8
Redundancy	4.53

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	unknown *	7.7 *		pH 7.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	1	protein	15-25 (mg/ml)
2	1	1	phosphate	50 (mM)
3	1	2	ammonium sulfate	2.0-2.2 (M)
4	1	2	Tris-HCl	50 (mM)
5	1	2		50 (mM)
6	1	2		0.001 (%(w/v))