2JCR
The hyaluronan binding domain of murine CD44 in a Type B complex with an HA 8-mer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | BRUKER AXS MICROSTAR |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-04 |
Detector | BRUKER |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 30.800, 82.100, 32.400 |
Unit cell angles | 90.00, 118.00, 90.00 |
Refinement procedure
Resolution | 28.610 - 2.000 |
R-factor | 0.179 |
Rwork | 0.175 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2jcq |
RMSD bond length | 0.009 |
RMSD bond angle | 1.280 |
Data reduction software | PROTEUM |
Data scaling software | SADABS |
Phasing software | MOLREP |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 28.600 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.110 | 0.240 |
Number of reflections | 9641 | |
<I/σ(I)> | 10.4 | 3.6 |
Completeness [%] | 92.3 | 67.5 |
Redundancy | 2.5 | 1.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8 | CO-CRYSTALS OF THE CD44/HA8 COMPLEX WERE PREPARED AFTER ADDITION OF HA8 (2MM FINAL CONCENTRATION) TO THE 0.5 MM PROTEIN SOLUTION FOLLOWED BY MIXING 1:1 WITH WELL SOLUTIONS CONTAINING 25% (W/V) PEG 3350 AND 100MM NACL IN 100MM HEPES BUFFERED AT PH 8.0 |