2JCL
Crystal structure of alpha-1,3 Galactosyltransferase (R365K) in the absence of ligands
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-06-09 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 95.930, 103.260, 121.490 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.810 - 3.290 |
| R-factor | 0.208 |
| Rwork | 0.205 |
| R-free | 0.28000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1k4v |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.544 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.420 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.200 | 0.620 |
| Number of reflections | 18881 | |
| <I/σ(I)> | 7.6 | 2.45 |
| Completeness [%] | 99.8 | 99.9 |
| Redundancy | 11.7 | 96 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | 0.3M SODIUM CACODYLATE BUFFER, PH 6.5, 1.4M SODIUM ACETATE, 2% DIOXANE |
