2JCG

Apo form of the catabolite control protein A (ccpA) from bacillus megaterium, with the DNA binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	CCD
Detector	MARRESEARCH
Spacegroup name	P 61 2 2
Unit cell lengths	74.450, 74.450, 238.840
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.600 - 2.600
R-factor	0.234
Rwork	0.229
R-free	0.29700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sxg
RMSD bond length	0.010
RMSD bond angle	1.300
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC (5.3.0022)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.700
High resolution limit [Å]	2.600	2.600
Rmerge	0.050	0.440
Number of reflections	14161
<I/σ(I)>	24.8	3.7
Completeness [%]	98.6
Redundancy	6.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8		3% PEG 3000, 100 MM CACL2, 10% (V/V) GLYCEROL, 100 MM TRIS-HCL PH 8.0